Molecular Phylogenetic Tracking of Tomato Leaf Curl Virus (ToLCV) Strain in the Philippines

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How do you control tomato leaf curl virus?

Spread petroleum jelly or Biotac on it. The yellow colour attracts the whiteflies to the boards and they stick to them. The boards are placed at the height of the plants. Monitor all crops, not just tomato plants as the whitefly may have passed the virus onto another crop.

To trace the possible route of the outset of the Tomato Leaf Curl Virus (ToLCV) in the Philippines, the phylogenetic relationships of ToLCV strains in the regions of epidemicity in the Philippines and other countries were investigated. The phylogenetic markers tagged as C1RAPG for ToLCV was specifically investigated to target the replication-associated protein of ToLCV was generated.

Phylogenetic analysis indicated that the TolCV strains in the Philippines segregate into two groups: Members of group 1 that include Ilocos Norte, Southern Luzon, and from the islands of Visayas and Mindanao are related to China, Malaysia, and Italy. While group 2 formed a monophyletic cluster with strain isolates from South Korea, Africa, Israel, and the USA implicating that the samples involved in this study are related to the Tomato Yellow Leaf Curl Virus (TYLCV) ancestor of ToLCV from the strains of Israel.

Tomato (Lycopersicum esculentum Mill) is one of the most popular and most widely grown fruit vegetable in the world. In the Philippines, the local tomato farming sector has been one of the major contributors to agriculture next to the onion. Tomato ranks fourth among the major vegetables and root crops in the total volume of production which has reached 91.26 thousand MT, 4.1 percent higher than the previous year's output amounting to 87.68 thousand MT.

Tomato yellow leaf curl virus (TYLCV) is one of the most well-known tomato-infecting begomoviruses and transmitted by Bemisia tabaci. Seed transmission has previously been reported for some RNA viruses, but TYLCV has not previously been described as a seed-borne virus.

Methodology
Samples collected, both symptomatic and asymptomatic were all suspected infected with TolCV. A total of ninety-four (94) samples which comprised 50 tomato leaves, 13 whiteflies, 21 soil and 10 water samples used were purposively obtained from different organic farms.

Various phylogenetic and detection studies demonstrate success in utilizing the genes selected for the study. In this work, the genes were selected as candidate genetic markers for screening of ToLCV infection among the prospective samples. The target sequences remained conserved in position and size hence rendering it ideal for molecular phylogenetic and diagnostic investigations.

Seed transmission was also possible and also detected in seeds and their seedlings of TYLCV-infected tomato plants that were infected by both viruliferous whitefly-mediated transmission and agro-inoculation. Taken together, ToLCV or TYLCV can also be transmitted not only via the insect vector but the seeds and the soil as a source of infection.

For further queries feel free to contact us, authors who want to submit the manuscript related to these subject related Tomato Leaf Curl Virus (ToLCV); Phylogenetic Tracking; Tomato Yellow Leaf Curl Virus (TYLCV) you can directly submit it through mail plantres@pathologyinsights.org (or) plantpathol@scholarlypub.com

Best Regards,
Editorial Team
Research Journal of Plant Pathology